Creatine amidinohydrolase

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August undefined, 2024

WebCreatinase (C2-AE) Enzymes for Clinical Chemistry The enzyme is useful for the determination of creatinine and creatine in clinical analysis. SPECIFICATION … WebCreatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride. Synonyms Creatine amidohydrolase; Creatinase; EC 3.5.3.3 Research & Diagnostic Related Protocols Related Reading

Thermostable creatine amidinohydrolase and process for …

WebMay 24, 2001 · Creatine amidinohydrolase is an enzyme which has a catalytic action of hydrolyzing creatine to generate sarcosine and urea. Creatine amidinohydrolase can be used for measuring the amount of creatine existing in human serum or urine, and so this enzyme can be used as a diagnostic agent for various diseases including renal diseases. WebThis enzyme system has been isolated in a cell-free extract and is composed of two separate enzymes. The first, creatinine hydrolase, interconverts creatinine and creatine to form an equilibrium mixture of the two. The second enzyme, creatine amidinohydrolase, splits creatine into equimolar amounts of sarcosine and urea. container store kennesaw ga

Creatinine hydrolase and creatine amidinohydrolase: I.

WebCreatinase (creatine amidinohydrolase, EC 3.5.3.3), a homodimer of 45 kDa subunit molecular mass, shows only limited functional stability, and is inaccessible to reconstitution after preceding deactivation, denaturation and dissociation. The enzyme has been characterized regarding its native and den … WebCreatine Phosphate, Disodium Salt, CAS 19333-65-4, is a high-energy phosphate compound that stores energy from mitochondria whenever sufficient amounts of ATP are … WebCreatininase from microorganisms Synonym (s): Creatinine Amidohydrolase CAS No.: 9025-13-2 EC No.: 232-786-8 Match Criteria: Product Name All Photos ( 1) Cholesterol … effects of ableism

Creatine amidinohydrolase and process for producing the same

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WebThe systematic name of this enzyme class is creatine amidinohydrolase. This enzyme participates in arginine and proline metabolism. Form. Liquid or lyophilized powder. Enzyme Commission Number. EC 3.5.3.3. CAS No. 37340-58-2. Storage. Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC. WebMay 2, 2010 · Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the … effects of abortion on mental healthWebMay 2, 2010 · Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. container store kansas city mo

"WebThe enzymatic assay for creatinine involves a series of coupled enzymatic reactions including creatininase enzymatic conversion of creatinine into the product creatine which itself is converted to sarcosine by creatine amidinohydrolase (creatinase), followed by oxidation of sarcosine by sarcosine oxidase (SOD) producing hydrogen peroxide. " - Creatine amidinohydrolase

Creatine amidinohydrolase

WebMar 15, 2024 · Name:CREATINE AMIDINOHYDROLASE,CAS:37340-58-2.Use:The concentration of creatinine in human serum is one of the important indicators of renal function. Enzymatic methods have been proposed in place of chemical assays. Because of the high specificity of enzymatic reaction, it can greatly improve the reliability of the … Creatinase is used as a key enzyme for enzymatic measurement of creatinine which catalyzes the hydrolysis of creatine to sarcosine and urea. It has been found in various kinds of bacteria such as Flavobacterium, Pseudomonas, Arthrobacter and Bacillus [6, 28, 30,31,32]. In this work, we analyzed the … See more In order to provide an initial characterization of the optimal temperature for wild-type afCR (WT), we first examined its activity across temperatures ranging … See more Based on the report of a I304L/F395V double mutant variant of creatinase from Erwinia with lower KM than its wild type enzyme [38], we first … See more To assess the kinetic stability of the variants, activity was measured across a range of temperatures (35–70 °C) to determine the temperatures at which enzyme activity was … See more

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WebTo create a potentiometric creatinine sensor, creatinine iminohydrolase, which catalyzes the production of ammonia from creatinine, can be immobilized on the surface of an … WebThe creatine is converted to sarcosine and urea by creatine amidinohydrolase. The sarcosine, in the presence of sarcosine oxidase, is oxidized to glycine, formaldehyde, …

WebThis enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this … WebMar 15, 2024 · CREATINE AMIDINOHYDROLASE - Introduction Chinese name: creatine enzyme English name: Creatinase Enzyme classification E C:3.5.3.3 Enzyme isoelectric …

WebENZYME entry: EC 3.5.3.3. creatinase. creatine amidinohydrolase. All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc. All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.5.3.-. All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.5.-.-. WebThe creatine is converted to sarcosine and urea by creatine amidinohydrolase. The sarcosine, in the presence of sarcosine oxidase, is oxidized to glycine, formaldehyde, …

WebCreatine amidinohydrolase In an early application, an enzyme electrode system was reported for the determination of creatinine and creatine, using a combination of creatinine amidohy-drolase, creatine amidinohydrolase and sarcosine oxidase, co-immobilized on an asymmetric cellulose acetate membrane.

WebThe enzyme creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine. Continuous use biosensors are becoming … container store keyboard trayWebSep 1, 2002 · The enzyme creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine. Continuous use biosensors are becoming more important in the ... effects of a broken noseWebOct 1, 1979 · Abstract. Creatinine amidohydrolase [EC 3.5.2.-; creatininase] was purified in an overall yield of 11% from cell-free extract of Pseudomonas putida var. naraensis, … effects of a brain bleedWebJul 1, 1982 · Abstract. A new enzymatic method is described for the determination of creatine in serum and urine using creatine amidinohydrolase (EC 3.5.3.3), sarcosine oxidase (EC 1.5.99.1) and formaldehyde dehydrogenase (EC 1.2.1.1). The principle of the method is as follows. Creatine is degraded to sarcosine and urea, and the sarcosine … container store keyboardWebThe enzymatic assay for creatinine involves a series of coupled enzymatic reactions including creatininase enzymatic conversion of creatinine into the product creatine … container store kids shoe rackWebThis enzyme system has been isolated in a cell-free extract and is composed of two separate enzymes. The first, creatinine hydrolase, interconverts creatinine and … container store keystoneWebCreatine Amidinohydrolase CAS Number: 37340-58-2 Enzyme Commission number: 3.5.3.3 ( BRENDA, IUBMB) EC Number: 253-471-1 MDL number: MFCD00130875 NACRES: NA.54 Pricing and availability is not currently available. Properties biological source bacterial (Actinobacillus spp.) Quality Level 200 form lyophilized powder specific … effects of a broken marriage on children