Hieffclone

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August undefined, 2024

WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into BL21 (DE3) competent cells. The correct clones were screened by primer P7/P8 (Table I) and colony PCR protocol in the above steps. inoculated into 3 ml fresh LB WebCN114134170A CN202411457529.2A CN202411457529A CN114134170A CN 114134170 A CN114134170 A CN 114134170A CN 202411457529 A CN202411457529 A CN 202411457529A CN 114134170 A CN114134170 A CN 114134170A Authority CN China Prior art keywords mcs product pgl primer nos Prior art date 2024-12-02 Legal status …

Gene editing of Duchenne muscular dystrophy using

WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into … WebHieffClone®ZeroTOPO-TASimpleCloningKit不含多克隆酶切位点(MCS)，在引入后续酶切位点进行酶切操作时，不会受到 MCS位点的影响，从而增加酶切效率以及克隆成功率。 … how to store bottle brush trees

A Selective Small‐Molecule c‐Myc Degrader Potently Regresses …

WebRESEARCH ARTICLE T E of I C and S R I P for C A10 Min Wang1 • Jingjing Yan1 • Liuyao Zhu1 • Meng Wang1 • Lizhen Liu1 • Rui Yu1 • Ming Chen1 • Jingna Xun1 • Yuling Zhang1 • Zhigang Yi1 • Shuye Zhang1 Received: 27 September 2024/Accepted: 24 December 2024/Published online: 6 March 2024 WebHieff Clone® Plus Multi One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任何载体的任何位点，可高效克隆50 bp-10 kb片 … WebHieffClone®ZeroTOPO-BluntCloningKit零背景TOPO-Blunt平末端克隆试剂盒 10909ES20 20T 625.00 产品描述本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA … read the villainess flips the script

Europe PMC - A Selective Small-Molecule c-Myc Degrader …

HieffClone™O_价格-品牌-详情介绍_丁香通

Web6 de mar. de 2024 · The cloning backbone PL451 and the three segments of CVA10 carrying overlapping end (15–20 bp) were amplified and seamlessly linked together in the transformed E. coli by recombination according to HieffClone TM MultiOneStep Cloning kit (YEASEN, Shanghai, China). how to store bootsWeb1 de dez. de 2024 · Introduction. Mutations Duchenne muscular dystrophy (DMD) is hereditary disease carried by the X chromosome, which driven by the mutation in DMD gene [1,2].DMD gene encodes Dystrophin protein that maintains to support and stabilize the structure and function of muscle tissue. The mutation of DMD gene lead to an absence … read the us constitution and amendments

"Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID … " - Hieffclone

Hieffclone

Web丁香通为您提供HieffClone零背商品详情介绍：价格：￥530，货号：10907ES20，品牌：Yeasen翌圣，产地：上海，详见丁香通HieffClone零背商品详情页； Web1 de jun. de 2024 · this digested vector by a HieffClone Plus multi One-Step . Cloning Kit (10912ES10, Y esen, China). The T urboID . fragment was amplified by PCR from a commercial gene

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Web产品描述. 本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需1-5分钟即可完成 … WebHieffClone óUniversalOneStepCloningKit 02 Component 1~3fragments 4~6fragments Negativecontrol ddH2O Upto20μL Upto20μL Upto20μL ...

Web20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c-Myc (L297A, A321del, L333A, R346A, S347A, … Web丁香通为您提供HieffClone™P商品详情介绍：价格：￥410，货号：10912ES，品牌：Yeasen翌圣，产地：上海，详见丁香通HieffClone™P商品详情页；丁香通丁香园

Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID fragment was amplified by PCR from a commercial gene (USA126049; Addgene, Watertown, MA, USA). The sequences of the primers were listed in Table S1. Web翌圣生物以工具酶为核心,研发生产销售pcr,qpcr,高保真酶,热启动酶,一步法快速克隆试剂盒,一步法快速支原体检测试剂盒等分子、蛋白、细胞、免疫等3000多种试剂及试剂盒,现有科 …

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Web15 de dez. de 2024 · Avian leukosis virus subgroup J disease (ALV-J) is a contagious and immunosuppressive avian disease caused by ALV-J virus. Although miRNA participate in various biological processes of tumors, little is known about the potential role of miRNA in ALV-J. Our previous miRNA and RNA sequencing data showed that the expression of … how to store bottles after sterilisingWeb12 de nov. de 2024 · 一种pbiflu荧光标签表达载体的制备方法及其应用技术领域 1.本发明属于分子生物学技术领域，具体涉及一种pbiflu荧光标签表达载体的制备方法及其应用。背景技术： 2.黄色荧光蛋白(yellow fluorescent protein，yfp)是将绿色荧光蛋白(gfp)第203为苏氨酸突变体为色氨酸，可以稳定生色团在激发状态的偶极矩 ... read the vagrant online freehttp://www.labbase.net/Supply/SupplyItems-4428942.html read the villainess is a marionette onlineWeb1 de dez. de 2024 · The enzymes using in molecular cloning experiment were Sap Ⅰ (NEB, USA), T4 DNA Ligase (Takara, Japan) and 2 × Hieffclone Enzyme Mix (YEASEN, … how to store bottled meadWeb丁香通为您提供HieffClone™O商品详情介绍：价格：￥1 - 1680，货号：10905ES25，品牌：进口/国产，产地：德国/中国，详见丁香通HieffClone™O商品详情页； how to store bottled waterWeb10 de abr. de 2024 · Hieff CloneTM Plus One Step Cloning Kit是一款简单、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任意载体的任意位点，可高效 … read the villainess is a marionette novelWeb20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c‐Myc (L297A, A321del, L333A, R346A, S347A, E351A, V361A, and Q365A) were generated using Hieff Mut Site‐Directed Mutagenesis Kit (YEASEN, 11003ES10). read the voodoo doctress manga free now