Thp-1 transfection lentivirus

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August undefined, 2024

WebTransfection protocol. In 1.5 mL tubes, mix 600 μL OptiMEM with 30μL Fugene. (NOTE: Do not allow the Fugene to come in contact with the tube in its undiluted form.) Incubate for … WebTransfection of THP-1 Cells Use this procedure to transfect plasmid DNA into THP-1 cells in a 24-well format (for other formats, see Scaling Up or Down Transfections, below). All …

Lentivirus Infection Protocol for stable cell line ... - GeneMedi

WebMar 2, 2024 · The lipofectamine 3000 and LTX transfection for virus production was combined with concentrating the resulting lentivirus supernatant by means of an … WebFeb 7, 2024 · Max Planck Institute for Infection Biology. I put 5x10^5 cells in 1 ml RPMI (10% FCS, P/S) in a 6-well plate and add 500 µl of virus supernatant (guess this depends on … lina line

THP-1 AP1-LUC2 - TIB-202-AP1-LUC2 ATCC

WebProtocol for Lentiviral Infection and Selection. Day 1: Plate target cells and incubate at 37°C, 5% CO 2 overnight. Day 2: Target cells should be approximately 70% confluent. Change to … WebThis protocol is for transfection in a 6 cm plate. The protocol can be scaled to produce different amounts of virus as needed. Day 1: For each plasmid to be transfected, plate … lina lovaton trujillo

Spinoculation and retronectin highly enhance the gene …

Viral transduction of cells using ‘spinfection’ – Besserer Lab

WebMar 21, 2016 · Filter 1 mL of lentiviral particle–containing supernatant from Step 11 through a 0.45-μm ... we use 70 μg of total DNA (1:1:1 (wt/wt/wt) ratio) and a total transfection … WebSuggestion: Add 1-10 µl lentivirus per 1,000-5,000 cells. Spin transduction in a desktop centrifuge (e.g. Sorvall RT6000) at 1,000 × g for 60 min at room temperature helps … lina kostenko poesia le aliWebAug 16, 2016 · THP-1 cells are recalcitrant to cationic- and lipid-based methods of transfection, meaning I was unable to use a Lipofection protocol. Protocol for recombinant Cas9 NLS Nuclease protein to edit the genome of a THP-1 cell line: 1. Add 1 ug of Cas9 nuclease preloaded with 250 ug of validated (in HEK293M) sgRNA to form the … lina matta pharmd

"WebDownload this protocol as PDF. 1. Background and purpose of the procedure Lentiviral transduction is an effective method for creating a stable cell line with a DNA cassette of … " - Thp-1 transfection lentivirus

Thp-1 transfection lentivirus

Lentivirus vector system, lentivirus production and transduction

WebNov 23, 2024 · The transfection rates were evaluated by a fluorescent microscope. Absolute qPCR for viral titration. Cell supernatant containing viral particles was collected at 1-, 2-, … Web1. Brief Description Lentiviral transduction is an effective method for creating a stable cell line with a DNA cassette of interest integrated into its genomic DNA, e.g. an sgRNA or …

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WebAug 16, 2016 · THP-1 cells are recalcitrant to cationic- and lipid-based methods of transfection, meaning I was unable to use a Lipofection protocol. Protocol for … WebLentivirus Protocol Download. This protocol is for the stable cell line construction based on puromycin selection. Day 1: Seed target cells in 24-well plates. The number of seeding …

WebCas9/shRNA/sgRNA/ORF Large Scale Lentiviral Production (T-175 flasks) 11 Feb 2024 pdf: 4: Viral Titering Protocol (alamarBlue) 21 Sep 2016 pdf: 5: Puromycin, Blasticidin and … WebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder …

WebMay 31, 2009 · Green fluorescent protein was expressed at various levels in different cells. In case of THP-1 monocytes cell viability was usually above 80% after transfection and transfection efficiency was more than 50%, whereas THP-1 macrophages showed a viability of around 75% and a transfection efficiency of more than 40% ( Fig. 1 C and D). http://www.protocol-online.org/biology-forums/posts/28348.html

WebCreation of a THP-1 cell line via stable transfection of a lentivirus vector containing LILRB5. WANG Xi-di;ZHANG Zhi-yong;REN ... LILRB5 was transfected in 293 Tcells and THP …

WebSummaryAutomatic TranslationSeptember 2nd, 2014. This protocol presents an efficient and reliable method to transfect human THP-1 macrophages with siRNA or plasmid DNA … lina launeWebIn conclusion, we have demonstrated the ability of VSV-pseudotyped lentiviral vectors to stably silence the PI3K p110α isoform in the monocytic cell lines THP-1 and U-937. Using … lina merlin la mia vitaWebUse this procedure to transfect plasmid DNA into THP-1 cells in a 24-well format (for other formats, see Scaling Up or Down Transfections, below). All amounts and volumes are given on a per well basis. The day of transfection, count the cells to determine culture density. Plate 1 x 10 5 cells per well lina omarWebRampart Bioscience. Aug 2024 - Present2 years 9 months. Monrovia, California, United States. • Screen candidate constructs and lentiviral vector (LVV) by transfection and … lina on tourWebMar 3, 2024 · THP-1 cells were purchased from ATCC, cultured in RPMI 1640 with 10% FBS and supplemented with 0.05 mM β-mercaptoethanol (β-ME). To generate M0 cells, ... lina molloholliWebMar 1, 2003 · We studied the transduction of primary human B lymphocytes and myeloma cells with lentiviral vectors. In peripheral blood B cells that had been activated with helper … lina mossWebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase … lina ruske